منابع مشابه
Analytical ultracentrifugation: sedimentation velocity and sedimentation equilibrium.
Analytical ultracentrifugation (AUC) is a versatile and powerful method for the quantitative analysis of macromolecules in solution. AUC has broad applications for the study of biomacromolecules in a wide range of solvents and over a wide range of solute concentrations. Three optical systems are available for the analytical ultracentrifuge (absorbance, interference, and fluorescence) that permi...
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In 1957, Meselson, Stahl, and Vinograd 1 introduced the remarkable technique of sedimentation equilibriuln in a density gradient for the study of the properties of high molecular weight DNA in solution. Because of the very high molecular weights of DNA, conventional sedimentation equilibrium cannot be used; for even at the lowest centrifugal fields which are practical, the force on these molecu...
متن کاملDetermination of membrane protein molecular weights and association equilibrium constants using sedimentation equilibrium and sedimentation velocity.
Regulated molecular interactions are essential for cellular function and viability, and both homo- and hetero-interactions between all types of biomolecules play important cellular roles. This chapter focuses on interactions between membrane proteins. Knowing both the stoichiometries and stabilities of these interactions in hydrophobic environments is a prerequisite for understanding how this c...
متن کاملEquilibrium sedimentation of turnip yellow mosaic virus.
Sedimentation equilibrium was achieved with turnip yellow mosaic virus at low speeds (600 rpm) in a magnetic ultracentrifuge. The experiments were carried out in the newly installed constant-speed rotor, equipped with automatic control and an electromagnetic drive. A particle mass of 5.55 x 10(6) daltons was calculated for the virus at vanishing concentrations, in essential agreement with the e...
متن کاملNon-equilibrium pattern selection in particle sedimentation
In this paper, we explain some well known experimental observations in fluid solid interaction from a thermodynamic perspective. In particular we use the extremum of the rate of entropy production to establish the stability of specific patterns observed in single and multiparticle sedimentation in an infinite fluid and the sedimentation of spheres in the presence of walls. While these phenomena...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1961
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.47.11.1848